Journal of Scientific Dentistry
Volume 10 | Issue 2 | Year 2020

Knowledge of Stem Cells among Pregnant Women in Rural Areas of Puducherry

Santha D Arumugam1, Joice Vinodine2, Balavaishnavi Guna3, Parameshwari Gobinathan4

1–4Department of Oral and Maxillofacial Pathology and Oral Microbiology, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Puducherry, India

Corresponding Author: Santha D Arumugam, Department of Oral and Maxillofacial Pathology and Oral Microbiology, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Puducherry, India, Phone: +91 9443634624, e-mail: drsantha73@gmail.com

How to cite this article Arumugam SD, Vinodine J, Guna B, Gobinathan P. Knowledge of Stem Cells among Pregnant Women in Rural Areas of Puducherry. J Sci Dent 2020;10(2):32–33.

Source of support: Nil

Conflict of interest: None


Stem cells are unique cells, as they have the potential to undergo repair and regeneration into a variety of distinct cell types of the body. These cells are of great importance in today’s regenerative medicine and can be isolated from specific tissues of the body including oral cavity. Especially, stem cells obtained from the placenta and umbilical cord that were considered to be biologic waste can be used in the treatment of cancerous conditions also. Moreover, the recent past has plenty of studies carried out in pulpal stem cells from the dental tissues. Hence, the present study was conducted to assess the level of awareness and knowledge about different sources of stem cells and its importance among expectant mothers in rural areas of Puducherry population. Through this study, the knowledge of stem cells will be emphasized among the pregnant women.

Keywords: Dental stem cells, Pregnant women, Stem cells..


Stem cells possess the ability to repair and regenerate into several cell types in the body, such as tooth, bone, cartilages, skin, adipose tissues, and glands. Isolation of these stem cells can be done from certain tissues within body, including oral cavity, and the blood in the umbilical cord is the richest source of stem cells. The stem cells are of two main types: the embryonic stem cells and the adult stem cells. Embryonic stem cells are otherwise known as somatic stem cells that represent undifferentiated cells present throughout the body after embryonic development, which can undergo multiplication through the process of cell division thereby replenish the dying cells and leading to regeneration of the damaged tissues. These stem cells are obtained from certain tissues of the body, including oral cavity, out of which the umbilical cord blood has its own importance, as it contains rich source of stem cells. Until recently, the cord blood that is retained in the placenta and the umbilical cord present after birth was considered as medical waste, and now the importance of umbilical cord has been reached to that level of even treating the life-threatening malignant disease.


To assess the knowledge about stem cells among expectant mothers in rural areas of Puducherry population.


The study participants consisted of pregnant women who visited the medical and dental hospital. It was a questionnaire-based study with 10 questions (9 questions related to knowledge and one question related to attitude).


S. no.Question no.Percentage
1Know about stem cells60
2Use of stem cells98
3Stem cell therapy95
4Collection of stem cells92
5Stem cells from umbilical cord92
6Stem cells from tooth34
7Stem cell banks35
9Storage and donation88
10To know more88


Stem cells are unspecialized cells although immature have the potential to develop into different cell lineages by differentiation.1 Their ability for self-renewal and differentiation makes them play a vital role in the repair of tissues and organs.2 Recently, studies reveal that the oral tissues serve as a rich source of stem cells. In dentistry, regeneration of the missing tissues in the oral cavity has been the main focus. Adult stem cells also called as somatic or postnatal stem cells are present in various tissues and organs. Recent studies in dentistry have revealed various sources for stem cells within the oral and maxillofacial region that reside in special site of each tissue called the stem cell niche. The adult stem cells in dental tissues are mainly of two types, namely, epithelial stem cells and mesenchymal-like cells.1 There are various sources of dental stem cells in orofacial region such as stem cells of dental follicle, stem cells of dental pulp, stem cells of apical papilla, periodontal ligament stem cells, progenitor cells of tooth germ, and immature dental pulp stem cells.3 The niche is present in the cervical loop of tooth apex which differentiates into enamel-producing cells, the ameloblasts. Regeneration of mesenchymal stem cells like periodontal tissues and dental pulp can form reparative dentin.1

The human periodontal ligament contain post-natal multipotent stem cells that serve as reservoir of stem cells and can be expanded ex vivo.4 The replacement of missing teeth in patients by the development of bioengineered teeth was possible because of stem cell technologies.5 The cultured tooth bud cells can give rise to these bioengineered teeth. Plenty of multipotent immature stem cells are present in deciduous teeth, which can form dentin-like structures although not a complete dentin–pulp complex. These tooth developed through bio-engineering technologies exhibit well-defined odontoblasts, predentin, dentin, and pulp chambers. It shows stellate reticulum, stratum intermedium, ameloblasts, dental enamel as well as putative Hertwig’s root sheath epithelium, thus morphologically mimicking structures of the enamel organ.6

The dental stem cells first obtained from dental pulp were dental pulp stem cells (DPSCs) isolated by enzymatic digestion of the pulp tissue of the human-impacted third molars. DPSCs can differentiate into adipocyte, chondrocyte, and osteocyte cell lineages, show expression for epithelial markers characteristically similar to neural stem cells. They have the ability to differentiate into vascular endothelial or neural cells in vitro.7 After oral surgery procedures, the human dental tissues are disposed considering them as medical waste, but these dental tissues are easy to obtain and are rich source of adult mesenchymal stem cells. The “medicine from disposed tissues” referred as “waste medicine” is a branch of regenerative medicine.8

Dental stem cell banking refers to the process of stem cell storage acquired from deciduous teeth and wisdom teeth of patients, which reflects the ability of dental stem cell-based regenerative therapy.9 Tissues containing stem cells can be acquired from patients and cryopreserved for several years to retain their regenerative capacity. These dental stem cells are well tolerated by the immune system and can be isolated from the cryopreserved tissue/tooth for future regenerative therapies when required.10


This questionnaire study revealed an average level (60%) of awareness about stem cells among pregnant women. This urges a need to spread more knowledge about the advances in applications, storage, banking and guidelines related to dental stem cells. Similar surveys and research must be conducted at different levels to spread the awareness globally so that everyone can gain the benefits in the future.


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