Citation Information :
Chandrasekar G, Arumugam SD, Nagaraj V, Premlal K, Muthanandam S, Rajaram S. Comparison of Efficacy of Candida Isolation from Oral Swab and Oral Rinse in HIV-infected Individuals. 2020; 10 (1):1-2.
Introduction: The major cause for morbidity and mortality in human immunodeficiency virus (HIV)-infected patient is fungal infection, which affects the quality of life. The most common fungal opportunistic infection is oral candidiasis. Almost all HIV patients are infected with Candida and 90–95% develop clinically, as the viral disease progresses. High plasma HIV RNA and low CD4 count are found to be associated with carriage of oral candidiasis in HIV patients. When HIV patients are treated with antiretroviral therapy, oropharyngeal candidiasis also subside simultaneously without specific antifungal therapy. The present study is intended to isolate Candida from oral swab and oral rinse and to find out which of these technique would demonstrate more isolation of Candida species.
Materials and methods: Samples are collected from 84 HIV seropositive patients attending tertiary care in Puducherry by using swab and rinse. Oral swabbing is done in areas with candidiasis and individuals without candidiasis. The oral rinse technique involves the patient holding 10 mL of sterile phosphate-buffered saline (0.01 M, pH 7.2) in the mouth for 1 minute. Collected samples are inoculated in Sabouraud\'s dextrose agar (SDA) at 37°C for 48 hours.
Results: Out of 84 samples collected, 40 (47.6%) were positive for Candida and 44 (52.4%) were negative. Out of which swab isolated 30 (75%) Candida and oral rinse 26 (65%) of Candida with p value 0.46.
Conclusion: In our study, oral swab yield more positive colonies compared to concentrated oral rinse; however, the p value was statistically significant.
Maheshwari M, Kaur R, Chadha S. Candida species prevalence profile in HIV seropositive patients from a major tertiary care hospital in New Delhi, India. J Pathogens 2016;2016:6204804.
Manikandan C, Amsath A. Isolation and rapid identification of Candida species from the oral cavity. J Pure Appl Zool 2013;1(2):172–177.
Vignesh Kanna B, Kumar A, Swapna M, Easow JM. Isolation and identification of candida species from various clinical samples in a tertiary care hospital. Int J Res Med Sci 2017;5(8):3520.
Khadka S, Sherchand JB, Pokhrel BM, et al. Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal. BMC Res Notes 2017;10(1):218. DOI: 10.1186/s13104-017-2547-3.
German advisory committee blood, subgroups assessment of pathogen: Human Immunodeficiency virus. 2016 may 9: 203–222.
Turner BG, Summers MF. Structural biology of HIV. J Mole Bio 1999;285(1):1–32. DOI: 10.1006/jmbi.1998.2354.
Swanstrom R, Coffin J. HIV-1 pathogenesis: the virus. Cold Spring Harb Perspect Med 2012;2(12):a007443. DOI: 10.1101/cshperspect.a007443.
Naif HM. Pathogenesis of HIV infection. Infect Dis Rep 2013;5(1):e6. DOI: 10.4081/idr.2013.s1.e6.
Rajendran R, Sivapathasundaram B. Shafer's textbook of oral pathology. 5th ed., Elsevier; 2006.
Turner SA, Butler G. The Candida pathogenic species complex. cold spring harbor perspectives in medicine. 2014;4(9):a019778. DOI: 10.1101/cshperspect.a019778.
Frimpong P, Amponsah EK, Abebrese J, Kim SM. Oral manifestations and their correlation to baseline CD4 count of HIV/AIDS patients in Ghana. J Korean Assoc Oral and Maxillofac Surg 2017;43(1):29–36. DOI: 10.5125/jkaoms.2017.43.1.29.
Xavier TF, Auxilia A, Kannan M. Isolation and characterization of UTI pathogens from HIV positive patients of Karur District, Tamil Nadu, India. Int J Curr Microbio Appl Sci 2015;4(1):558–563.
Egusa H, Soysa NS, Ellepola AN, Yatani H, Samaranayake LP. Oral candidiasis in HIV-infected patients. Curr HIV Res 2008;6(6):485–499. DOI: 10.2174/157016208786501445.
Kamtane S, Subramaniam A, Suvarna P. A comparative study of oral candidal carriage and its association with CD4 count between HIV-positive and healthy individuals. J Int Assoc Provid AIDS Care 2013;12(1):39–43. DOI: 10.1177/1545109711423444.
Coronado-Castellote L, Jiménez-Soriano Y. Clinical and microbiological diagnosis of oral candidiasis. J Clin Experiment Dent 2013;5(5):e279. DOI: 10.4317/jced.51242.