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VOLUME 13 , ISSUE 1 ( January-June, 2023 ) > List of Articles

ORIGINAL RESEARCH

Mesotherapy Using Vitamin C and Platelet Rich Plasma as an Anti-inflammatory Agent in Persistent Gingival Inflammation: A Pilot Study

Gaurav Singh, Shailendra S Chauhan, Aditya Sinha, Satendra Sharma

Keywords : Anti-inflammatory agents, Inflammation, Mesotherapy, Periodontal therapy, Platelet-rich plasma, Vitamin C

Citation Information : Singh G, Chauhan SS, Sinha A, Sharma S. Mesotherapy Using Vitamin C and Platelet Rich Plasma as an Anti-inflammatory Agent in Persistent Gingival Inflammation: A Pilot Study. 2023; 13 (1):3-6.

DOI: 10.5005/jp-journals-10083-1027

License: CC BY-NC 4.0

Published Online: 14-07-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Abstract

In mesotherapy, the recipient receives minor dosages of biologically active substances in an area of pathology, which are delivered to the skin via local intradermal therapy (LIT). In addition to their anti-inflammatory properties, vitamin C and platelet-rich plasma (PRP) can improve tissue repair and reduce inflammation. Aim: This study examined whether local injections of vitamin C and PRP could be used to treat persistent gingival inflammation. Materials and methods: Following phase-I therapy, 15 patients with chronic persistent gingival inflammation underwent PRP and vitamin C injections respectively into the gingiva. Each patient underwent a weekly evaluation to see if the inflammation had subsided. Results: During recall visits, the inflammation of the injected sites showed a significant improvement in post clinical pictures. Conclusions: Local intradermal therapy (LIT) with vitamin C and PRP has a synergistic interaction along with scaling and root planing in the treatment of persistent gingival inflammation.

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  1. Eid AH. Impact of melanin pigmentation on gingival infammation histopathological and biochemical analysis. Egypt Dent J 2012;58(2):1289–1294. ISSN: 0070-9484.
  2. El-Taukhy MA, Salama SM, Abou-Shousha SA, Ismail SS, Saleh M. Effects of chronic ethanol and vitamin C administration on production of tumor necrosis factor-alpha and interleukin-6 in rats. Egypt J Immunol 2006;13(1):1–10. PMID: 17974145.
  3. Shiga M, Kapila YL, Zhang Q, Hayami T, Kapila S. Ascorbic acid induces collagenase-1 in human periodontal ligament cells but not in Mc3t3-E1 osteoblast-like cells: Potential association between collagenase expression and changes in alkaline phosphatase phenotype. J Bone Miner Res 2003;18(1):67–77. DOI: 10.1359/jbmr.2003.18.1.67.
  4. Zanoni JN, Lucas NM, Trevizan AR, Souza ID. Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid. An Acad Bras Cienc 2013;85(1):327–35. DOI: 10.1590/s0001-37652013005000003.
  5. Özaslan M, Aytekin T, Kiliç IH, Bozkurt AI, Güdür E, Cengiz B, et al. The effect of vitamin C supplementation on leucocyte counts and exercise performance. J Exerc Physiol Online 2004;7(2):101–105. ISSN: 1097-9751.
  6. Aguirre R, May JM. Inflammation in the vascular bed: Importance of vitamin C. Pharmacology Ther 2008;119(1):96–103. DOI: 10.1016/j.pharmthera.2008.05.002.
  7. Schilephake H. Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 2002;1(5):469–484. DOI: 10.1054/ijom.2002.0244.
  8. Giannobile WV. Periodontal tissue engineering by growth factors. Bone 1996;19(Suppl 1):23s–37s. DOI: 10.1016/s8756-3282(96)00127-5.
  9. Tischler M. Platelet rich plasma. The use of autologous growth factors to enhance bone and soft tissue grafts. N Y State Dent J 2002;68(3):22–24. PMID: 11989332.
  10. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85(6):638–646. DOI: 10.1016/s1079-2104(98)90029-4.
  11. Brahmavar B, Khan SF, Khan AM. Mesotherapy using vitamin C as an anti-Inflammatory agent in persistent gingival inflammation: A case series. J Indian Soc Periodontol 2021;25(3):203–206. DOI: 10.4103/jisp.jisp_443_20.
  12. Kutlu B, Tiğlı Aydın RS, Akman AC, Gümüşderelioglu M, Nohutcu RM. Platelet-rich plasma-loaded chitosan scaffolds: Preparation and growth factor release kinetics. J Biomed Mater Res B Appl Biomater 2013;101(1):28–35. DOI: 10.1002/jbm.b.32806.
  13. Löe H. The gingival index, the plaque index and the retention index systems. J Periodontol 1967;38(6):610–616. DOI: 10.1902/jop.1967.38.6.610.
  14. Muhlemann HR, Son S. Gingival sulcus bleeding a leading symptom in initial gingivitis. Helv Odontol Acta 1971;15(2):107–113. PMID: 5315729.
  15. Yussif NM, Abdul Aziz MA, Abdel Rahman AR. Evaluation of the anti- inflammatory effect of locally delivered vitamin C in the treatment of persistent gingival inflammation: Clinical and histopathological study. J Nut Metal 2016;2016:2978741. DOI: 10.1155/2016/2978741.
  16. Mammucari M, Gatti A, Maggiori S, Bartoletti CA, Sabato AF. Mesotherapy, definition, rationale and clinical role: A consensus report from the Italian society of mesotherapy. Eur Rev Med Pharmacol Sci 2011;15(6):682–694. PMID: 21796873.
  17. Mackay D, Miller AL. Nutritional support for wound healing. Altern Med Rev 2003;8(4):359–377. PMID: 14653765.
  18. Sørensen LT, Toft BG, Rygaard J, Ladelund S, Paddon M, James T, et al. Effect of smoking, smoking cessation, and nicotine patch on wound dimension, vitamin C, and systemic markers of collagen a metabolism. Surgery 2010;148(5):982–990. DOI: 10.1016/j.surg.2010.02.005.
  19. Mohammed BM, Fisher BJ, Huynh QK, Wijesinghe DS, Chalfant CE, Brophy DF, et al. Resolution of sterile inflammation: Role for vitamin C. Mediators Inflame 2014;2014:173403. DOI: 10.1155/2014/173403.
  20. Daniels A, Jefferies S. Analysis of capacity of novel, antioxidant tooth paste, small population clinical study: Comparison to levels of gingival inflammation reduction reported in historical control and therapeutic tooth brushing studies. Clinical research and biomaterials analysis and review report, CS Bioscience, New York; 2007.
  21. Shimabukuro Y, Nakayama Y, Ogata Y, Tamazawa K, Shimauchi H, Nishida T, et al. Effects of an ascorbic acid–derivative dentifrice in patients with gingivitis: A double-masked, randomized, controlled clinical trial. J Periodontol 2015;86(1):27–35. DOI: 10.1902/jop.2014.140138.
  22. Pinnell SR, Yang H, Omar M, Riviere Nm, Debuys HV, Walker LC, et al. Topical L-ascorbic acid: Percutaneous absorption studies. Dermatol Surg 2001;27(2):137–142. DOI: 10.1046/j.1524-4725.2001.00264.x.
  23. Bahal P, Djemal S. Dental erosion from an excess of vitamin C. Case Rep Dent 2014;2014:485387. DOI: 10.1155/2014/485387.
  24. Terranova VP, Odziemiec C, Tweden KS, Spadone DP. Repopulation of dentin surfaces by periodontal ligament cells and endothelial cells. Effect of basic fibroblast growth factor. J Periodontol 1989;60(6):293–301. DOI: 10.1902/jop.1989.60.6.293.
  25. Takayama S, Murakami S, Miki Y, Ikezawa K, Tasaka S, Terashima A, et al. Effects of basic fibroblast growth factor on human periodontal ligament cells. J Periodontal Res 1997;32(8):667–675. DOI: 10.1111/j.1600-0765.1997.tb00577.x.
  26. Geiger F, Bertram H, Berger I, Lorenz H, Wall O, Eckhardt C, et al. Vascular endothelial growth factor gene-activated matrix (Vegf165- Gam) enhances osteogenesis and angiogenesis in large segmental bone defects. J Bone Miner Res 2005;20(11):2028–2035. DOI: 10.1359/JBMR.050701.
  27. Gamal AY, Mailhot JM. The effect of local delivery of Pdgf-Bb on attachment of human periodontal ligament fibroblasts to periodontitis-affected root surfaces in vitro. J Clin Periodontol 2000;27(5):347–353. DOI: 10.1034/j.1600-051x.2000.027005347.x.
  28. Appay V, Brown A, Cribbes S, Randle E, Czaplewski LG. Aggregation of rantes is responsible for its inflammatory properties. Characterization of nonaggregating, noninflammatory rantes mutants. J Biol Chem 1999;274(39):27505–27512. DOI: 10.1074/jbc.274.39.27505.
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