Introduction: The probability of infection during dental practice is extremely significant, particularly when utilizing water from the dental chair's water boosters, which is often provided directly to the patient. As a result, there is a pressing need to create a unique disinfection composition for use in dental treatments, particularly in water lines. Aim: The aim of this study is to examine and compare the efficacy of a novel composition based on hypochlorous acid (HOCl) vs sodium hypochlorite in preventing cross-infection during dental treatment operations. Materials and methods: Patients with moderate-to-severe gingivitis were randomly assigned to one of three groups of five. They had been designated for ultrasonic scaling. Group I (control): For ultrasonic scaling, freshly prepared reverse osmosis (RO) water was utilized in the dental unit water booster. Group II (test group 1): As a disinfectant in the dental unit water booster, sodium hypochlorite (100 ppm) was utilized. Group III (test group 2): A novel (100 ppm)-based composition was used as a disinfectant in the dental unit water booster. The water units’ oxidation-reduction potential (ORP) after scaling was also assessed. Results: Postoperatively, all three groups exhibit a decrease in oxidation-reduction potential. Group II has a significant decrease in ORP (p = 0.001). Conclusion: This study found that using sodium hypochlorite as an ultrasonic liquid coolant considerably decreases the ORP during scaling when compared to RO water and HOCl. Clinical significance: As an ultrasonic liquid cooler, sodium hypochlorite considerably decreases the ORP during scaling.