Age estimation plays an important role in determining the identity of an unknown individual in case of any crime or mass disaster and is not only needed in case of dead individuals but also for living persons for record purpose. Aims and objectives: The study was done to identify the age of an individual using the exfoliated cells of buccal mucosa by comparing the cell size. Materials and methods: Buccal smears were obtained using a wet wooden spatula by gentle scraping motion, fixed using 95% ethanol, and were stained using standard hematoxylin and the eosin staining procedure. The average cell size was measured using a stage micrometer grid. The statistical analysis of the data was done using Chi-square and Kruskal-Wallis tests. Results: The results show the statistical significance with p < 0.001 correlating the decrease in cell size with increasing age. Conclusion: Age-related changes are observed in buccal smears that can be used as a tool for age estimation.
Shetty DC, Wadhwan V, Khanna KS, Jain A, Gupta A. Exfoliative cytology: a possible tool in age estimation in forensic odontology. J Forensic Dent Sci 2015;7(1):63–66. DOI: 10.4103/0975-1475.150321.
Schmeling A, Geserick G, Reisinger W, Olze A. Age estimation. Forensic Sci Int 2007;165(2–3):178–181. DOI: 10.1016/j.forsciint.2006.05.016.
Juneja M, Devi YB, Rakesh N, Juneja S. Age estimation using pulp/tooth area ratio in maxillary canines-A digital image analysis. J Forensic Dent Sci 2014;6(3):160–165. DOI: 10.4103/0975-1475.137047.
Reddy VS, Kumar GS, Vezhavendhan N, Priya S. Cytomorphometric analysis of normal exfoliative cells from buccal mucosa in different age groups. Int J Clin Dent Sci 2011;2(3):53–56.
Joseph CC, Reddy BH, Cherian NM, Kannan SK, George G, Jose S. Intraoral digital radiography for adult age estimation: a reliable technique. J Indian Acad Oral Med Radiol 2013;25(4):287–290.
Montogomery PW. A study of exfoliative cytology in normal human oral mucosa. J Dent Res 1951;30(1):12–18. DOI: 10.1177/00220345510300010501.
McConnell G. A Manual of Pathology, 3rd ed., Philadelphia: W. B. Saunders Company; 1915; p. 56.
Bibbo M. Comprehensive cytopathology, 2nd ed., Philadelphia: W. B. Saunders and Company; 1997.
Koss LG. The future of cytology. The Wachtel lecture for 1988. Acta Cytol 1990;34(1):1–9.
Anuradha A, Sivapathasundharam B. Image analysis of normal exfoliated gingival cells. Indian J Dent Res 2007;18(2):63–66. DOI: 10.4103/0970-9290.32422.
Cowpe JG, Longmore RB, Green MW. Quantitative exfoliative cytology of normal oral squames: an age, site and sex-related survey. J R Soc Med 1985;78(12):995–1004. DOI: 10.1177/014107688507801204.
Patel PV, Kumar S, Kumar V, Vidya G. Quantitative cytomorphometric analysis of exfoliated normal gingival cells. J Cytol 2011;28(2):66–72. DOI: 10.4103/0970-9371.80745.
Eid RA, Sawair F, Landini G, Saku T. Age and the architecture of oral mucosa. Age (Dordr) 2012;34(3):651–658. DOI: 10.1007/s11357-011-9261-1.
Nallamala S, Venkateswara Rao Guttikonda PK, Taneeru S. Age estimation using exfoliative cytology and radiovisiography: a comparative study. J Forensic Dent Sci 2017;9(3):144–148. DOI: 10.4103/jfo.jfds_39_16.