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VOLUME 4 , ISSUE 2 ( July-December, 2014 ) > List of Articles


Expression of Bax in Oral Lichen Planus – An Immunohistochemical Study

Aparna K, Santha Devy A

Keywords : Oral Lichen Planus, Apoptosis, Bax, Immunohistochemistry

Citation Information : K A, A SD. Expression of Bax in Oral Lichen Planus – An Immunohistochemical Study. 2014; 4 (2):3-8.

DOI: 10.5005/jsd-4-2-3

License: CC BY-NC 4.0

Published Online: 03-08-2020

Copyright Statement:  Copyright © 2014; The Author(s).


Background: Oral lichen planus (OLP) affect a minor percentage of the general population, but the morbity caused by this disease is a cause of concern. Apoptosis is known to occur during the course of OLP. Aims and Objectives: To find the expression of Bax using immunohistochemistry and To find its role in the pathogenesis of oral lichen planus. Methodology: The present study was done using Immunohistochemical procedure on the 21 samples procured from the archival material. The pro-apoptotic marker Baxwas demonstrated and analysed using peroxidase – antiperoxidaseimmunostainingmethod. Results: The results were analysed using the positivity of the immunostaining of the samples. Further the positivity was graded in terms of their staining intensity, distribution and location of stain uptake. The results were subjected to statistical analysis and analysed using a non-parametric analysis, Mann Whitney test and was found to be statistically significant with p value of 0.000. Conclusion: The results obtained in this study suggest that the process of apoptosis occurs in OLP. Hence inhibition of apoptosis in the patients could reduce the severity of the lesions and thus representing new specific targets for treatment of Lichen Planus.

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  1. P.B. Sugerman, N.W. Savage, L.J. Walsh, Z.Z. Zhao, X.J. Zhou, A. Khan, G.J. Seymour, M. Bigby. The pathogenesis of oral lichen planus. CROBM 2002;13:350.
  2. Riikka Mattila, Stina Syrjänen. Caspase cascade pathways in apoptosis of oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:618-623.
  3. Hedberg N, Ng A, Hunter N. A semi-quantitative assessment of the histopathology of oral lichen planus. J Oral Pathol 1986; 15:268-72.
  4. Shimizu M, Higaki Y, Higaki M, Kawashima M. The role of granzyme B–expressing CD8-positive T cells in apoptosis of keratinocytes in lichen planus. Arch Dermatol Res 1997;289:527-32.
  5. Bertram JS. The molecular biology of cancer. Mol Aspects Med 2000;21:167-223.
  6. Peter ME, Heufelder AE, Hengartner MO. Advances in apoptosis research. Proc Natl Acad Sci U S A 1997;94:12736-7.
  7. Bramanti TE, Dekker NP, Lozada-Nur F, Sauk JJ, Regezi JA. Heat shock (stress) proteins and gamma delta T lymphocytes in oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995; 80:698-704.
  8. Chaiyarit P, Kafrawy AH, Miles DA, Zunt SL, Van Dis ML, Gregory RL. Oral lichen planus: an immunohistochemical study of heat shock proteins (HSPs) and cytokeratins (CKs) and a unifying hypothesis of pathogenesis. J Oral Pathol Med 1999; 28:210-215.
  9. Neppelberg E, Johannessen AC, Jonsson R. Apoptosis in oral lichen planus. Eur J ral Sci 2001;109:361-4.
  10. Kim SG, Chae CH, Cho BO, Kim HN, Kim HJ, Kim IS, Choi JY. Apoptosis of oral epithelial cells in oral lichen planus caused by upregulation of BMP-4. J Oral Pathol Med 2006;35:37-45.
  11. Dekker NP, Lozada-Nur F, Lagenaur LA, MacPhail LA, Bloom CY, Regezi JA. Apoptosis-associated markers in oral lichen planus. J Oral Pathol Med 1997;26:170-5.
  12. Tanda N, Mori S, Saito K, Ikawa K, Sakamoto S. Expression of apoptotic signaling proteins in leukoplakia and oral lichen planus: quantitative and topographical studies. J Oral Pathol Med 2000;29:385-93.
  13. Bloor BK, Malik FK, Odell EW, Morgan PR. Quantitative assessment of apoptosis in oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:187-95.
  14. Bascones-Ilundain C, Gonzalez-Moles MA, Campo-Trapero J, et al. No differences in caspase-3 and Bax expression in atrophic-erosive vs. reticular oral lichen planus. J Eur Acad Dermatol Venereol 2008;22:204-12.
  15. Crispian Scully, Marco Carrozzo. Oral mucosal disease: Lichenplanus. British Journal of Oral and Maxillofacial Surgery 2008;46:15–21.
  16. Imavati Budihardjo, Holt Oliver, Michael Lutter, Xu Luo, Xiaodong Wang. Biochemical Pathways of Caspase Activation Duration Apoptosis. Annu. Rev. Cell Dev. Biol 1999;15:269-90.
  17. Matthews JB, Scully CM, Potts AJ. Oral lichen planus: an immunoperoxidase study using monoclonal antibodies to lymphocyte subsets. Br J Dermatol 1984;111:587-595.
  18. C. Kantari, H. Walczak. Caspase-8 and Bid: Caught in the act between death receptors and mitochondria. Biochimica et Biophysica Acta 2011;1813:558–563.
  19. Fernando A.C.G. de Sousa, Thais C. Paradella, Yasmin R, Carvalho, Luiz E B Rosa. Immunohistochemical expression of PCNA, p53, bax and bcl-2 in oral lichen planus and epithelial dysplasia. Journal of Oral Science 2009;51(1):117-121.
  20. Xu'e CHEN, Yan WU, Jiawen LI, Zhixiang LIU, Qing YUE, Houjun LIU. Expression of Apoptosis Related Protein in Skin Lesions of Lichen Planus and Its Implication. J Huazhong Univ Sci Technol [Med Sci] 2008;28(3):349-351.
  21. Shima Nafarzadeh, Sina Jafari, Ali Bijani. Assessment of Bax and Bcl-2 Immunoexpression in Patients with Oral Lichen Planus and Oral Squamous Cell Carcinoma. IJMCM 2013;2(3):136-142.
  22. Yamamoto T, Osaki T. Characteristic cytokines generated by keratinocytes and mononuclear infiltrates in oral lichen planus. J Invest Dermatol 1995; 104:784-788.
  23. A. Basu and S. Haldar. The relationship between Bcl2, Bax and p53: consequences for cell progression and cell death. Molecular Human Reproduction 1998;4(12):1099. 1109.
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