Introduction: The major cause for morbidity and mortality in human immunodeficiency virus (HIV)-infected patient is fungal infection, which affects the quality of life. The most common fungal opportunistic infection is oral candidiasis. Almost all HIV patients are infected with Candida and 90–95% develop clinically, as the viral disease progresses. High plasma HIV RNA and low CD4 count are found to be associated with carriage of oral candidiasis in HIV patients. When HIV patients are treated with antiretroviral therapy, oropharyngeal candidiasis also subside simultaneously without specific antifungal therapy. The present study is intended to isolate Candida from oral swab and oral rinse and to find out which of these technique would demonstrate more isolation of Candida species. Materials and methods: Samples are collected from 84 HIV seropositive patients attending tertiary care in Puducherry by using swab and rinse. Oral swabbing is done in areas with candidiasis and individuals without candidiasis. The oral rinse technique involves the patient holding 10 mL of sterile phosphate-buffered saline (0.01 M, pH 7.2) in the mouth for 1 minute. Collected samples are inoculated in Sabouraud's dextrose agar (SDA) at 37°C for 48 hours. Results: Out of 84 samples collected, 40 (47.6%) were positive for Candida and 44 (52.4%) were negative. Out of which swab isolated 30 (75%) Candida and oral rinse 26 (65%) of Candida with p value 0.46. Conclusion: In our study, oral swab yield more positive colonies compared to concentrated oral rinse; however, the p value was statistically significant.