Aim: To assess the knowledge and attitudes levels on regenerative endodontic procedures among dental residents in and around Puducherry. Materials and methods: It is a descriptive cross-sectional questionnaire-based study carried out in the region of in and around Puducherry in 2020. One hundred and ninety-three participants from five dental colleges included in this study: Adhiparasakthi Dental College and Hospital, n = 27; Mahatma Gandhi Postgraduate Institute of Dental Sciences, n = 26; Raja Muthiah Dental College and Hospital, n = 36; Indira Gandhi Institute of Dental Sciences, n = 54; and Sri Venkateswara Dental College and Hospital, n = 55. A Questionnaire through the Google Form comprised a set of 18 questions. Results: A total of 82.4% participants were aware of different types of regenerative endodontic procedures; 55.4% of the participants gained knowledge from curriculum; 28% of them gained knowledge through conference and journals; and 47.2% of them were aware of the different types of stem cells, of which only very few were able to identify all the possible cell sources (26.4%) and possible teeth sources (20.7%) from which the dental stem cells can be extracted. Conclusion: Regenerative therapies are being one among the most novel topics, ruling the world of medicine and dentistry (7), it necessitates the inclusion of intensive training and more numbers of continuing dental education (CDE) programs to be conducted to uplift the standard of knowledge among the dental residents.